Keck Foundation Updates
In the summer of 2017, enrichment work targeting the growth of uncultivated thermoalkaliphilic Bacteria and Archaea yielded several promising cultures. Enriched populations were visualized using Fluorescent in situ Hybridization (FISH), detecting the possible presence of several novel bacterial populations. These enrichments are currently being sequenced to identify specific species and their relative abundance. Progress has also been made on extracting proteins and metabolites from thermoalkaline YNP hot springs. This work will provide important insights into proteins that allow thermoalkaliphilic organisms to grow and survive under such extreme conditions. Finally, metagenomic datasets generated from several high pH hot springs are currently being assembled, and will help in identifying potential metabolic pathways employed by thermoalkaline microbes, thus allowing for genomics-enabled cultivation efforts.
Sampling trip in Yellowstone